Different Recipes

SM Agar (1 Liter)

Peptone 10 grams
Yeast Extract 1 gram
Glucose 10 grams
KH2PO4 1.9 grams
K2HPO4 1.3 grams
MgSO4anhydrous .49 grams
Agar 17 grams

** SM Broth is without Agar

HL5 Medium (1 Liter)

Peptone 14 grams
Yeast Extract 7 grams
KH2PO4 0.5 grams
Na2HPO4 0.5 grams
Glucose 31.5 grams

LB-Broth Miller (1Liter)

Tryptone 10 grams
Yeast Extract 5 grams
NaCl 10 grams
Final PH 7.0 ± 0.2

KK2 buffer (1 liter)

KH2PO4 2.2 grams
K2HPO4 .7 grams


Starving Buffer (100 mls)

Na2HPO4 1.78 grams
KH2PO4 9.9 grams

**Water Agar Plates are 1% for example for 300ml add 3 grams of Agar

Starving Plates (1 Liter)

Starving Buffer 20 mls
H2O 980 mls
Agar 20 grams

Hay Plates (1 Liter)

KH2PO4 1.5 grams
Na2HPO4 .62 grams
Agar 15 grams


TBE (1X)

Tris Base 216 grams
Boric Acid 110 grams
Na2EDTA 18.6 grams
ddH2O 20 Liters


LP Agar (1 Liter)

Lactose 1 gram
Bactopeoptone 1 gram
Agar 15 grams


SS ( 1 Liter)

NaCl 0.6 grams
KCL 0.75 grams
CaCl2 0.3 grams


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